We use the oxDNA coarse-grained model to provide a detailed characterization of the fundamental structural properties of DNA origami, focussing on archetypal 2D and 3D origami. The model reproduces well the characteristic pattern of helix bending in a 2D origami, showing that it stems from the intrinsic tendency of anti-parallel four-way junctions to splay apart, a tendency that is enhanced both by less screened electrostatic interactions and by increased thermalmotion. We also compare to the structure of a 3D origami whose structure has been determined by cryo-electronmicroscopy. The oxDNA average structure has a root-mean-square deviation from the experimental structure of 8.4A° , which is of the order of the experimental resolution. These results illustrate that the oxDNA model is capable of providing detailed and accurate insights into the structure of DNA origami, and has the potential to be used to routinely pre-screen putative origami designs and to investigate the molecular mechanisms that regulate the properties of DNA origami.
|Data di pubblicazione:||2019|
|Titolo:||Coarse-grained modelling of the structural properties of DNA origami|
|Rivista:||NUCLEIC ACIDS RESEARCH|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/nar/gky1304|
|Appare nelle tipologie:||2.1 Articolo su rivista |