ARCA è l'archivio istituzionale ad accesso aperto della ricerca dell'Università Ca' Foscari Venezia e nasce nel 2014 con lo scopo di raccogliere, diffondere e conservare la produzione scientifica dell'Università. Costituisce di fatto il catalogo della ricerca di Ateneo.ARCA è un sistema Open Access: è non solo consultabile da tutti e visibile nei motori di ricerca, ma anche interoperabile con il portale cerCa. In questo modo vengono valorizzate la produzione scientifica dell'Ateneo e la visibilità dei ricercatori.
EnglishCardiac troponin T (TnT) is a highly sensitive cardiac biomarker for myocardial infarction. In this study, the fabrication and characterisation of a novel sensor for human TnT based on a molecularly-imprinted electrosynthesised polymer is reported. A TnT sensitive layer was prepared by electropolymerisation of o-phenylenediamine (o-PD) on a gold electrode in the presence of TnT as a template. To develop the molecularly imprinted polymer (MIP), the template molecules were removed from the modified electrode surface by washing with alkaline ethanol. Electrochemical methods were used to monitor the processes of electropolymerisation, template removal and binding. The imprinted layer was characterised by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and atomic force microscopy (AFM). The incubation of the MIP-modified electrode with respect to TnT concentration resulted in a suppression of the ferro/ferricyanide redox process. Experimental conditions were optimised and a linear relationship was observed between the peak current of [Fe(CN)6]3-/[Fe(CN)6]4- and the concentration of TnT in buffer over the range 0.009-0.8ng/mL, with a detection limit of 9pg/mL. The TnT MIP sensor was shown to have a high affinity to TnT in comparison with non-imprinted polymer (NIP) electrodes in both buffer and blood serum. © 2013 Elsevier B.V.
| Autori: | ||
| Data di pubblicazione: | 2013 | |
| Titolo: | An ultrasensitive molecularly imprinted human cardiac troponin sensor | |
| Rivista: | BIOSENSORS & BIOELECTRONICS | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/j.bios.2013.07.013 | |
| Volume: | 50 | |
| Appare nelle tipologie: | 2.1 Articolo su rivista |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| 3.pdf | Documento in Post-print | Accesso chiuso-personale | Riservato |
http://hdl.handle.net/10278/3717631
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