19F nmr relaxation proved to be a proper method to evaluate the Cu2+/Cu+ ratio at the active site of Cu, Zn superoxide dismutase in either equilibrium or turnover conditions. In the steady-state under fluxes of O2-, the enzyme was found to contain 50% Cu2+, in accord with the equal rates of copper catalytic reduction and oxidation. Previous results giving 75% Cu at the steady-state without change of the overall catalytic constant were confirmed for samples subjected to freeze-drying or freezing-thawing. © 1981 Academic Press, Inc.

19F relaxation as a probe of the oxidation state of Cu, Zn superoxide dismutase. Studies of the enzyme in steady-state turnover

ARGESE, Emanuele;
1981

Abstract

19F nmr relaxation proved to be a proper method to evaluate the Cu2+/Cu+ ratio at the active site of Cu, Zn superoxide dismutase in either equilibrium or turnover conditions. In the steady-state under fluxes of O2-, the enzyme was found to contain 50% Cu2+, in accord with the equal rates of copper catalytic reduction and oxidation. Previous results giving 75% Cu at the steady-state without change of the overall catalytic constant were confirmed for samples subjected to freeze-drying or freezing-thawing. © 1981 Academic Press, Inc.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in ARCA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10278/37637
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 21
  • ???jsp.display-item.citation.isi??? ND
social impact