In this research the production of polyhydroxyalkanoates (PHA) by activated sludge enriched in a sequencing batch reactor (SBR) at high organic load, has been investigated. The SBR was operated at four different organic load rates (OLR), in the range 8.5 - 40.8 gCOD/L/day, and hydraulic retention times (HRT) in the range 1 - 0.21 day, both being simultaneously varied by changing the cycle length from 2 h to 0.42 h. Both parameters affected the establishment of the feast and famine conditions needed for the selection of PHA-producing microorganisms. The highest observed values of polymer production rates and yields (∼ 400 mgCOD/gCOD/h and 0.53 COD/COD, respectively) were obtained at the lowest OLR investigated (8.5 gCOD/L/day), with a corresponding HRT of 1 day. Microbial community analysis, based on denaturing gradient gel electrophoresis (DGGE), revealed that hydraulic and organic loads also played a main role on the microbial speciation within the SBR. Copyright © 2010, AIDIC Servizi S.r.l.

Effect of hydraulic and organic loads in Sequencing Batch Reactor on microbial ecology of activated sludge and storage of polyhydroxyalkanoates

Valentino, Francesco;Majone, Mauro;
2010

Abstract

In this research the production of polyhydroxyalkanoates (PHA) by activated sludge enriched in a sequencing batch reactor (SBR) at high organic load, has been investigated. The SBR was operated at four different organic load rates (OLR), in the range 8.5 - 40.8 gCOD/L/day, and hydraulic retention times (HRT) in the range 1 - 0.21 day, both being simultaneously varied by changing the cycle length from 2 h to 0.42 h. Both parameters affected the establishment of the feast and famine conditions needed for the selection of PHA-producing microorganisms. The highest observed values of polymer production rates and yields (∼ 400 mgCOD/gCOD/h and 0.53 COD/COD, respectively) were obtained at the lowest OLR investigated (8.5 gCOD/L/day), with a corresponding HRT of 1 day. Microbial community analysis, based on denaturing gradient gel electrophoresis (DGGE), revealed that hydraulic and organic loads also played a main role on the microbial speciation within the SBR. Copyright © 2010, AIDIC Servizi S.r.l.
IBIC2010 2nd International Conference on Industrial Biotechnology
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10278/3736878
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