In this paper the voltammetric behaviour of the anticancer drug irinotecan (CPT-11), its injectable form in the clinical treatment regimen, irinotecan hydrochloride (CPT-11HCl), and its main metabolites (namely SN-38, SN-38G, APC, and NPC), and the natural chemical analogous camptothecin (CPT) were investigated in acetonitrile using a glassy carbon electrode (GCE), in view of developing an analytical protocol for the therapeutic drug monitoring (TDM) of CPT-11 in patients under chemotherapy regimens. Our results showed that all compounds provided rather complex cyclic voltammetric (CV) patterns in both the negative and positive potential regions. The overall results indicated that the processes recorded in the negative potential region at both GCE and Pt electrodes could be hardly exploited for TDM applications, because of the overlapping of the peaks. Instead, in the positive potential region, the oxidation of the piperidine moiety of CPT-11, which was obtained in CPT-11HCl acetonitrile solutions basified with Na2B4O7 (synthetic solutions), proved to be useful for irinotecan quantification, as its process at the GCE took place over a potential region essentially free from interference. Preliminary differential pulse voltammetry (DPV) measurements performed in synthetic solutions of CPT-11HCl, over the concentration range 0.2 – 9 µM, showed a linear dependence between peak current and concentration with a satisfactory correlation coefficient of 0.992. The reproducibility was within 5% from three replicates.

Voltammetric Behaviour of the Anticancer Drug Irinotecan and its Metabolites in Acetonitrile. Implications for Electrochemical Therapeutic Drug Monitoring

Bonazza, Gregorio;POLO, Federico;Salvatore Daniele
2018-01-01

Abstract

In this paper the voltammetric behaviour of the anticancer drug irinotecan (CPT-11), its injectable form in the clinical treatment regimen, irinotecan hydrochloride (CPT-11HCl), and its main metabolites (namely SN-38, SN-38G, APC, and NPC), and the natural chemical analogous camptothecin (CPT) were investigated in acetonitrile using a glassy carbon electrode (GCE), in view of developing an analytical protocol for the therapeutic drug monitoring (TDM) of CPT-11 in patients under chemotherapy regimens. Our results showed that all compounds provided rather complex cyclic voltammetric (CV) patterns in both the negative and positive potential regions. The overall results indicated that the processes recorded in the negative potential region at both GCE and Pt electrodes could be hardly exploited for TDM applications, because of the overlapping of the peaks. Instead, in the positive potential region, the oxidation of the piperidine moiety of CPT-11, which was obtained in CPT-11HCl acetonitrile solutions basified with Na2B4O7 (synthetic solutions), proved to be useful for irinotecan quantification, as its process at the GCE took place over a potential region essentially free from interference. Preliminary differential pulse voltammetry (DPV) measurements performed in synthetic solutions of CPT-11HCl, over the concentration range 0.2 – 9 µM, showed a linear dependence between peak current and concentration with a satisfactory correlation coefficient of 0.992. The reproducibility was within 5% from three replicates.
2018
289
File in questo prodotto:
File Dimensione Formato  
1-s2.0-S0013468618320802-main.pdf

non disponibili

Tipologia: Versione dell'editore
Licenza: Accesso chiuso-personale
Dimensione 2.38 MB
Formato Adobe PDF
2.38 MB Adobe PDF   Visualizza/Apri
1-s2.0-S0013468618320802-mmc1.pdf

non disponibili

Descrizione: Supporting information
Tipologia: Versione dell'editore
Licenza: Accesso chiuso-personale
Dimensione 1.39 MB
Formato Adobe PDF
1.39 MB Adobe PDF   Visualizza/Apri

I documenti in ARCA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10278/3706377
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 11
social impact