Ensembles of copper nanowire electrodes (CuWNEEs) are prepared via electrodeposition in track-etched polycarbonate membranes. Three different preparation methods are compared showing that the better results in terms of sensor durability and reproducibility are achieved by pre-sputtering a thin gold film on the templating membrane and attaching it to a supporting electrode by exploiting the adhesion property and ionic conductivity of a thin Nafion interlayer. SEM-EDS analyses together with double layer charging currentsmeasurements indicate thatthese arrays are formed by copper nanowires with 400 nmdiameter, 10 m length distributed with a spatial density of 1 × 108 nanowires/cm2. The voltammetric reduction of nitrate at CuWNEEs is characterized by a well-resolved cathodic peak at approximately −0.680V vs Ag/AgCl, whose current scales linearly with the nitrate concentration in the 10–400 M range. The limit of detection (LOD) achieved by simple linear sweep voltammetry is in the 1.7–3.0 M range, depending on the CuWNEE preparation method, such LOD values being among the lowest reported up to now in the literature. The possibility to use CuWNEEs in chloride and nitrite containing water samples is demonstrated.