Uptake from Water, Internal Distribution and Bioaccumulation of Selenium in Scenedesmus obliquus, Unio mancus and Rattus norvegicus: Part A

The Se-75 internal bioavailability was investigated in microalgae, mussels and rats as biological experimental models. The Se-75 accumulation from freshwater to microalgae [Scenedesmus obliquus (Turpin) Kutzing], from freshwater to mussels (Unio mancus Lamark) and, finally, per os to rats (Rattus norvegicus Berkenhout) was followed using Se-75-labelled selenite looking at Se-75 uptake, retention, intracellular distribution and binding with cellular biocomplexes. After exposure to 10, 50 and 500 mu g Se L-1, the microalgae showed an inhibitory effect on population growth only at the highest concentration. Mussels exposed to 105 mu g Se L-1 showed an accumulation of the element with time in all tissues. Intracellularly, Se was present in all subcellular fractions, especially in the cytosol. Rats were treated via oral administration with 5 mu g Se rat(-1). After 24 h, liver and kidney showed the highest Se concentration.

The 75Se internal bioavailability was investigated in microalgae, mussels and rats as biological experimental models. The 75Se accumulation from freshwater to microalgae [Scenedesmus obliquus (Turpin) Kützing], from freshwater to mussels (Unio mancus Lamark) and, finally, per os to rats (Rattus norvegicus Berkenhout) was followed using 75Se-labelled selenite looking at 75Se uptake, retention, intracellular distribution and binding with cellular biocomplexes. After exposure to 10, 50 and 500 μg Se L−1, the microalgae showed an inhibitory effect on population growth only at the highest concentration. Mussels exposed to 105 μg Se L−1 showed an accumulation of the element with time in all tissues. Intracellularly, Se was present in all subcellular fractions, especially in the cytosol. Rats were treated via oral administration with 5 μg Se rat−1. After 24 h, liver and kidney showed the highest Se concentration.