In this work, gold microelectrodes are employed as traps for the detection of volatilized metallic mercury produced by mercuric reductase (MerA) extracted from an Hg-resistant Pseudo monas putida strain FB1. The enzymatic reduction of Hg (II) to Hg (0) was induced by NADPH cofactor added to the samples. The amount of Hg(0) accumulated on the gold microelectrode surface was determined by anodic stripping voltammetry (ASV) after transferring the gold microelectrode in an aqueous solution containing 0.1 M HNO3 + 1 M KNO3. Electrochemical measurements were combined with spectrofluorometric assays of NADPH consumption to derive an analytical expression for the detection of a relative MerA activity of different samples with respect to that of P. putida. The method developed here was employed for the rapid determination of MerA produced by bacteria harbored in soft tissues of clams (Ruditapes philippinarum), collected in high Hg polluted sediments of Northern Adriatic Sea in Italy.
|Data di pubblicazione:||2012|
|Titolo:||A rapid Electrochemical Procedure for the Determination of Hg(0) Produced by Mercury-Reductase: Application for Monitoring Hg-resistant Bacteria Activity|
|Rivista:||ENVIRONMENTAL SCIENCE & TECHNOLOGY|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/es301444a|
|Appare nelle tipologie:||2.1 Articolo su rivista |