The aim of this study was to investigate the protective effect of Ferula hermonis roots and Silybum marianum seeds on Methidathion induced oxidative stress in Siganus rivulatus fish. Fish health status was evaluated through stress biomarkers (LPO, ALT, and LDH), in addition to total cholesterol. Normal and stressed fish tissue were subjected to histo-pathological investigation. Moreover, a holistic picture about all detectable metabolites including the known and unknown compounds in Ferula hermonis roots and Silybum marianum seeds was performed through HPLC-HRMS. LC50 of Methidathion for Siganus rivulatus fish was determined via bath route for 24 hours, and it was about 7.5ug/l. Our results showed a significant increase in the serum ALT, LDH activities, and lipid peroxidation products (MDA) in tissue homogenates (hepatopancreas, gills, and muscles) compared to control group. With exception to total cholesterol, which exhibit a significant decrease, in the toxin treated group. The histological observation of this study support our research about the protective effects of Ferula hermonis roots and Silybum marianum seeds on Methidathion induce oxidative stress in Siganus rivulatus fish. Our study provides a new insight into the potential alternative therapeutic solution to Aquaculture contamination by organophosphate insecticides in general.

Analytical and biological studies on the immunomodulatory potential of flavonoids in fish aquaculture / Al-Jawasreh, Raid Issa Mahmoud. - (2020 Feb 14).

Analytical and biological studies on the immunomodulatory potential of flavonoids in fish aquaculture

Al-Jawasreh, Raid Issa Mahmoud
2020-02-14

Abstract

The aim of this study was to investigate the protective effect of Ferula hermonis roots and Silybum marianum seeds on Methidathion induced oxidative stress in Siganus rivulatus fish. Fish health status was evaluated through stress biomarkers (LPO, ALT, and LDH), in addition to total cholesterol. Normal and stressed fish tissue were subjected to histo-pathological investigation. Moreover, a holistic picture about all detectable metabolites including the known and unknown compounds in Ferula hermonis roots and Silybum marianum seeds was performed through HPLC-HRMS. LC50 of Methidathion for Siganus rivulatus fish was determined via bath route for 24 hours, and it was about 7.5ug/l. Our results showed a significant increase in the serum ALT, LDH activities, and lipid peroxidation products (MDA) in tissue homogenates (hepatopancreas, gills, and muscles) compared to control group. With exception to total cholesterol, which exhibit a significant decrease, in the toxin treated group. The histological observation of this study support our research about the protective effects of Ferula hermonis roots and Silybum marianum seeds on Methidathion induce oxidative stress in Siganus rivulatus fish. Our study provides a new insight into the potential alternative therapeutic solution to Aquaculture contamination by organophosphate insecticides in general.
14-feb-2020
32
Scienze ambientali
Capodaglio, Gabriele
File in questo prodotto:
File Dimensione Formato  
956334-1217967.pdf

accesso aperto

Tipologia: Tesi di dottorato
Dimensione 3.3 MB
Formato Adobe PDF
3.3 MB Adobe PDF Visualizza/Apri

I documenti in ARCA sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10579/17843
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact